At the beginning of the study, all participants indicated what they eat and gave blood samples. Every two to three years, the researchers asked by post or telephone how the diet and health of the participants had changed. By 2005, about 800 participants developed type 2 diabetes and by 2006, about 550 developed cardiovascular disease. The team created a lipid profile of ceramides and dihydroceramides in the blood. The researchers then examined whether those ceramides that correlated with an increased risk of disease in the participants were dependent on their diet.
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