Production and Analysis of Cellulase Enzyme Diversity from the Genus Anoxybacillus – Universitas Airlangga Official Website

Biomass contains lignocellulose, whose cellulose and hemicellulose content is a renewable energy source (renewable energy). The bioconversion of cellulose to oligosaccharides and glucose requires the activity of the cellulase enzyme. Oligosaccharides and glucose are important energy sources for the health of the digestive system of animals. The source of the cellulase enzyme can come from a variety of biodiversity microorganisms, the unique biochemical properties of cellulase from the genus Anoxybacillus it is not yet fully available, although the nucleotide sequence of the coding gene can be accessed in GenBank. Bioinformatics technology is one technology in silico which reveals the biochemical properties and diversity of cellulase types in nature, especially from the genus Anoxybacillus. Appropriate biochemical properties can be useful for the application of cellulase in the bioconversion process of cellulose from biofuels from agro-industrial by-products to a new energy source that can increase the added value of animal feed . This type of enzyme is often called the lignocellulase group Exogenous nutritional enzyme.

Researchers have succeeded in isolating local isolates Anoxybacillus flavithermus TP-01 from the hot springs in Mount Pancar, Bogor, West Java. Anoxybacillus flavithermus TP-01 is thermophilic, bilaterally anaerobic, and produces cellulase enzymes. Analysis of diversity, biochemical properties and structure of different genes Anoxybacillus made by technology in silicoincluding SOPMA, SWISS-MODEL, I-TASSER, ProSA, and SAVES. The technology can reveal physicochemical properties, phylogenetic construction, functional analysis, alignment multiple rowsand predicted the secondary and tertiary structures of the cells of the species Anoxybacillus. The results show that the cellulase enzyme is thermostable and hydrophilic. These results provide new insight into the origin of cellulase Anoxybacillus flavithermus The local TP-01 will greatly support cellulose development applications in industry.

Author: Ni Nyoman Tri Puspaningsih

Detailed information from this research can be found in our article at:

Link: https://doi.org/10.13057/be/d250641

Rahmat Eko Sanjaya, Ni Nyoman Tri Puspaningsih, Ali Rohman, Hakiki Rahmasari, Rosli Md. Illias, Nawee Jantarit, Kazuhito Fujiyama, Andre Pratama, Fatiha Khairunnisa (2024). Production and diversity analysis of cells from the genus Anoxybacillus. Biodiversity 25:6, 2705-2718.

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