Rabies is still a widespread zoonotic disease in Indonesia. Although effective rabies vaccines have been developed and are available, improvements in vaccine design are still needed to increase their effectiveness and availability. Rabies is transmitted mainly through the bite of an infected animal, with dogs being the most common reservoir in Indonesia. Vaccination of pets and people is an important strategy for controlling the spread of the virus.
Plasma-based DNA vaccines have received considerable attention because of their ability to induce strong and long-lasting immune responses. In an effort to increase the effectiveness of the rabies vaccine, this research focuses on creating a plasmid-based DNA vaccine that targets the Indonesian RBV glycoprotein, with myeloid differentiation factor 88 (MyD88) on it. included as a genetic enhancer. The RBV glycoprotein is a key antigenic target for vaccine development, as it plays an important role in viral entry and is highly immunogenic.
In this study, we used computational biology techniques to elucidate the structural and functional interactions between a glycoprotein, MyD88, and host immune receptors, shedding light on the mechanism by which MyD88 enhances immunity to to promote vaccination. Using in silico methods, this research contributes to the rational design of plasmid-based DNA vaccines against rabies, with a particular focus on the Indonesian context. This approach has the potential to significantly reduce the number of rabies cases, increase vaccine effectiveness, and pave the way for experimental validation and future clinical trials.
Understanding the structural basis of the interaction between the glycoprotein, MyD88, and host immune receptors is essential. This knowledge provides important insight into the mechanisms underlying MyD88’s ability to enhance vaccine-induced immunity. By eliminating these structural interactions, we pave the way for more targeted and effective vaccine design strategies. It is important to consider structural aspects as we move forward with experimental validation. One important aspect of this study is the consideration of genetic variation in Indonesian RBV.
By taking into account the genetic diversity of viruses, we open up the possibility of personalized vaccine design. This unique approach has the potential to increase vaccine effectiveness, addressing the unique challenges of different virus variants in specific regions.
In addition, the inclusion of MyD88 in the vaccine construct can not only enhance the immune response against the targeted Indonesian RBV, but also has an effect on cross-protection against other RBV strains. The role of MyD88 in immune activation could lead to broader and stronger protection, particularly relevant in regions with multiple RBV variants.
Author: Teguh Hari Sucipto, Arif Nur Muhammad Ansori, Nelson Chandra, et al.
Detailed information about this scientific article can be found at: https://jmpcr.samipubco.com/article_193604.html
2024-10-04 02:14:00
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