Our team has developed an experimental method to generate immunosuppressive cells of monocyte origin called Human Monocyte-derived Suppressor Cells (HuMoSC). Although their immunosuppressive properties have already been shown, no study had yet evaluated their interest in tissue remodeling.The objective of this thesis was to evaluate the therapeutic potential of HuMoSCs and their supernatant for the treatment of tissue remodeling. On the other hand, we sought to identify the mechanisms of action involved in the functions of the supernatant of HuMoSC. The effect of HuMoSCs was first evaluated in giant cell arteritis using an ex vivo temporal artery culture model, and then in an in vitro and in vivo fibrosis model, similar to that described in Systemic Scleroderma.Our results showed that HuMoSC supernatant decreased the expression of matrix proteins and pro-fibrosing factors as well as cell proliferation.In conclusion, this thesis work has highlighted the anti-remodeling properties of HuMoSC supernatant thus representing a new hope in the treatment of fibrosing diseases.
Our team has developed an experimental method to generate immunosuppressive cells of monocytic origin called Human Monocyte-derived Suppressor Cells (HuMoSC). If their immunosuppressive properties have already been shown, no study had yet evaluated their benefits on tissue remodeling. The objective of this thesis was on the one hand, to evaluate the therapeutic potential of HuMoSCs and their supernatant for the treatment tissue remodeling. On the other hand, we sought to identify the mechanisms of action involved in the functions of the supernatant. The effect of HuMoSCs was first evaluated in giant cell arteritis using an ex vivo temporal artery culture model, then in an in vitro and in vivo fibrosis model, similar to that described in Scleroderma. Systemic. Our results showed that the supernatant of HuMoSC decreased the expression of matrix proteins and pro-fibrotic factors as well as cell proliferation. In conclusion, this thesis work highlighted the anti-remodeling properties of the supernatant of HuMoSC , thus representing new hope in the treatment of fibrosing diseases.
