Abstract
The growth characteristics, cytopathogenicity and viral polypeptides of the virulent strain XJ of Junin virus (JV), its attenuated derivative XJC13 and another naturally attenuated JV strain, IV4454, were comparatively studied. IV4454 and XJC13 viruses showed the highest and lowest cytopathology for Vero cells, respectively, as measured by plaque morphology, cell viability and inhibition of host cell protein synthesis. The kinetics and electrophoretic patterns of viral polypeptides in infected cell extracts were very similar among the three strains, whereas differences were detected in the surface glycoprotein GP38 by peptide mapping after limited proteolysis.
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Résumé
We have compared the growth traits, cytopathogenicity and viral polypeptides of the virulent XJ strain of Junin virus (VJ), its attenuated derivative XKC13 and another naturally attenuated strain of VJ, IV4454. Strains IV4454 and XJC13 have, respectively, the highest and lowest cytopathogenic power on Vero cells, in terms of plaque morphology, cell viability and inhibition of host cell protein synthesis. The kinetic and electrophoretic profiles of viral polypeptides in extracts from cells infected with the three strains are very similar. Differences concerning the surface glycoprotein GP38 were however detected by the mapping of the peptides, after partial proteolysis.
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Keywords
Junin virus
Arenaviridae
Virulence
Polypeptide
Strains
Cytopathogenicity
Attenuated virus
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Keywords
Junin virus
Arenaviridae
Virulence
Polypeptide
Strains
Cytopathogenic power
Attenuated virus
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Copyright © 1990 Published by Elsevier Masson SAS
