Errors in the DNA replication process create ‘stress’ that interferes
NSMF protein promotes phosphorylation by making weak proteins stronger
Contributing to the treatment of cancer, neurological and aging-related diseases
The photo has nothing to do with the content of the article. (Photo = pixabay)
(Seoul=Internal and external broadcasting) Just as we do not perform well under stressful situations during work, a study has found that DNA in the body can also cause various problems in stressful situations.
UNIST (Ulsan National Institute of Science and Technology) recently announced that “a research team led by professors Lee Jae-il, Kim Hong-tae, and Choi Jang-hyeon of the Department of Life Sciences found out the process by which NSMF protein solves problems that occur when DNA replication is stressed.”
NSMF protein is a protein that is mainly active in the brain and is involved in the development and movement of nerve cells related to growth, secretion of reproductive hormones, and olfaction.
If this protein does not function normally, it can lead to rare diseases such as Kallman syndrome with hypogonadism and loss of smell.
A mimetic diagram of the increase in phosphorylation of RPA (replication protein A) of ATR by NSMF under DNA replication stress. (Photo = UNIST)
The research team paid attention to the NSMF protein to relieve DNA replication stress.
A phenomenon in which replication is hindered by an error in the DNA replication process is called ‘DNA replication stress’, and the DNA structure of the protein that has stopped replication due to this is unwound from a double helix to a single helix.
‘Replication protein A’ binds to single-stranded DNA, and a phosphorylation process in which a phosphate group composed of phosphorus and oxygen is chemically attached to the combined replication protein A proceeds.
Phosphorylated replication protein A brings a protein that relieves replication stress to the site where replication has stopped, allowing it to activate normally.
The role of the NSMF protein is to separate the weakly bound replication protein A, making the remaining protein stronger.
The stronger protein meets the phosphorylated protein ‘ATR’ and strongly binds to replication protein A again, resulting in rapid phosphorylation.
The research team confirmed that DNA replication stress is quickly resolved through this principle.
(Upper row from left) Professor Janghyun Choi, Department of Biological Sciences at UNIST, Researcher Yeji Han.
Professor Lee said, “This study will be able to contribute to the treatment of cancer, neurological diseases, and aging-related diseases by identifying molecular mechanisms related to DNA replication stress relief.”
This research, in which Ph.D. student Kang Yu-jin participated as the first author, was conducted with support from the Samsung Foundation for Future Technology, etc., and was recently published online in the international journal ‘Nucleic Acids Research’ (Paper title: Alteration of replication protein A binding mode on single-stranded DNA by NSMF potentiates RPA phosphorylation by ATR kinase).
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2023-07-14 09:05:08
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